Journal: Molecular Metabolism

Article Title: Intestinal CREBH overexpression prevents high-cholesterol diet-induced hypercholesterolemia by reducing Npc1l1 expression

doi: 10.1016/j.molmet.2016.09.004

Figure Lengend Snippet: Changes in cholesterol-related gene expression patterns in intestine and liver of WT and CREBH Tg mice fed a LD . Intestinal and hepatic mRNA was extracted from WT and CREB Tg mice fed a LD for 2 weeks. (A) Expression of cholesterol-related genes in small intestine (n = 6–14 mice/group). (B) Intestinal protein was extracted from WT and CREBH Tg mice. Western blotting was performed with anti-NPC1L1 antibody. (C) Expression of genes related to lipid and bile acid metabolism in liver (n = 6–7/group). Results presented as mean ± SEM. ***p < 0.001, **p < 0.01, *p < 0.05.

Article Snippet: Protein expression levels of NPC1L1 were analyzed in small intestine lysates using a rabbit anti-NPC1L1 antibody (Novus Biologicals cat. NB400-127).

Techniques: Gene Expression, Expressing, Western Blot

Journal: Molecular Metabolism

Article Title: Intestinal CREBH overexpression prevents high-cholesterol diet-induced hypercholesterolemia by reducing Npc1l1 expression

doi: 10.1016/j.molmet.2016.09.004

Figure Lengend Snippet: CREB3L3 reduces Npc1l1 promoter activity . Caco-2 cells were co-transfected with a luciferase reporter plasmid containing segments of the mouse Npc1l1 promoter and a CREBH or empty expression plasmid. Forty-eight hours after transfection, the cells were harvested and luciferase assay was performed. (A) CREBH inactivated the −0.3 kbp Npc1l1 promoter luc in a dose-dependent manner. (B) The effects of CREBH and SREBP-2 on a series of Npc1l1 promoter luc vectors. (C) EMSA assay indicated that CREBH directly bound to the region from −32 to +5 bp of the Npc1l1 promoter. n = 3–4/group. ***p < 0.001, **p < 0.01, and *p < 0.05.

Article Snippet: Protein expression levels of NPC1L1 were analyzed in small intestine lysates using a rabbit anti-NPC1L1 antibody (Novus Biologicals cat. NB400-127).

Techniques: Activity Assay, Transfection, Luciferase, Plasmid Preparation, Expressing

Journal: Molecular Metabolism

Article Title: Intestinal CREBH overexpression prevents high-cholesterol diet-induced hypercholesterolemia by reducing Npc1l1 expression

doi: 10.1016/j.molmet.2016.09.004

Figure Lengend Snippet: CREBH knockout induces severe hypercholesterolemia in mice fed a LD . (A) Plasma cholesterol (n = 6–11/group), triglyceride (n = 10/group), and NEFA levels (n = 10/group) in WT and CREBH null mice fed a LD for 2 weeks. (B) Representative pictures of whole livers and. H&E stained liver sections from LD-fed WT and CREBH null mice. (C) Liver cholesterol and triglyceride levels (n = 6–10/group). (D) Intestinal cholesterol and triglyceride levels (n = 3–5/group). (E) Fecal cholesterol output (n = 4–5/group). (F) Expression of Npc1l1 in small intestine following normal chow diet or LD. Results presented as mean ± SEM. *p < 0.05.

Article Snippet: Protein expression levels of NPC1L1 were analyzed in small intestine lysates using a rabbit anti-NPC1L1 antibody (Novus Biologicals cat. NB400-127).

Techniques: Knock-Out, Clinical Proteomics, Staining, Expressing

Journal: Iranian Journal of Medical Sciences

Article Title: Effects of Saponin from Trigonella Foenum-Graecum Seeds on Dyslipidemia

doi:

Figure Lengend Snippet: ABCG8 and NPC1L1 protein expression in intestine. (2A) The protein bands of ABCG8, NPC1L1 and beta-actin. (2B) The data of the protein bands semi-quantitatively analyzed by QuantityOne. Data are reported as mean±SD of six rats carried out in duplicate. P values are obtained from the independent sample t-test between two groups (HFD group and another group). *P<0.05: Indicate significant difference from the HFD group; **P<0.01: Indicate extremely significant difference from the HFD group; ABCG8: ATP-binding cassette transporter G8, NPC1L1: Niemann-Pick C1-Like 1

Article Snippet: Membranes were cut into strips and blocked by 5% skim milk for 1 hour before incubating with Anti-ABCG8 antibody (sc-30111, Santa Cruz Ltd.), Anti-NPC1L1 antibody (TA309769, Origene Ltd.), and mouse anti-beta actin antibody monoclonal antibody (ZSGB-BIO Ltd.) at 4 °C overnight.

Techniques: Expressing, Binding Assay

Journal: Iranian Journal of Medical Sciences

Article Title: Effects of Saponin from Trigonella Foenum-Graecum Seeds on Dyslipidemia

doi:

Figure Lengend Snippet: The CYP7A1, ABCG8, NPC1L1, HMG-CoAR, SREBP-1c, ABCA1, and SR-BI mRNA expression in liver. Data are reported as mean±SD of eight rats carried out in duplicate. P values are obtained from the independent sample t-test between two groups (HFD group and another group). *P<0.05: Indicate significant difference from the HFD group; **P<0.01: Indicate extremely significant difference from the HFD group; ABCA1: ATP-binding cassette transporter A1; ABCG8: ATP-binding cassette transporter G8; CYP7A1: Cholesterol 7alpha-hydroxylase; HMG-CoAR: 3-hydroxy-3-methyl glutaryl coenzyme A reductase; NPC1L1: Niemann-Pick C1-Like 1; SR-BI: Scavenger receptor class B type I; SREBP-1: Sterol regulatory element-binding protein-1

Article Snippet: Membranes were cut into strips and blocked by 5% skim milk for 1 hour before incubating with Anti-ABCG8 antibody (sc-30111, Santa Cruz Ltd.), Anti-NPC1L1 antibody (TA309769, Origene Ltd.), and mouse anti-beta actin antibody monoclonal antibody (ZSGB-BIO Ltd.) at 4 °C overnight.

Techniques: Expressing, Binding Assay